Beyond microdialysis, behavioral, EEG, electrophysiology and bioanalytical services, we also offer a variety of other supplemental and complementary services and study types.
Our biotechnical team has many years’ experience designing and running ‘in-life’ procedures – often in combination with PK studies and other assays – and welcomes the opportunity to design a custom program suited to your specific needs and objectives.
Experimental modeling of neurodegenerative disease
Our client sought a novel experimental model to mimic a neurodegenerative disease that required precise intracranial delivery of a protein, followed by monitoring for several weeks.
With our expert team of surgeons, we can design and implement the best technical approach to deliver a pathogenic protein to a distinct brain region. We then monitor the animals for several weeks to determine subtle changes in behavior that are indicative of a progressing disease state.
Having and applying the technical expertise to provide precise targeting of small regions in the rodent brain.
We developed an experimental model of a neurodegenerative disease that was subsequently used to test for novel therapeutics against the disease.
Our biotechnical team’s deep experience with all types of in vivo study provides an array of dosing techniques, lesion models, and vector administration. As with all of our services, these surgical/compound administration techniques can be applied in combination with other studies like microdialysis, behavior or EEG measurements for greatest value and efficiency.
Techniques in rats and mice include:
- Oral dosing (PO)
- Intraperitoneal dosing (IP)
- Intravenous dosing (IV)
- Subcutaneous dosing (SC)
- Blood vessel cannulation (femoral, jugular, tail vein)
- Local tissue administration
- Intracerebral injections
- Intracerebroventricular (ICV)
- Local administration by brain perfusion
- IM administrations
Label-free Receptor Occupancy
Radio-label-free LC-MS/MS receptor occupancy studies make it possible to calculate the specific receptor occupancy of a test compound by comparing levels in an area rich in the target receptor with an area of the brain with very low target expression.
General PK studies
If you have an early stage compound, it’s important to know whether the compound is being absorbed following oral administration. Our complete pharmacokinetic studies include formulation evaluation and dosing all the way through to the final report. Often these are most valuable when deployed in combination with other techniques, such as behavior or neurochemistry, to develop a clear pharmacokinetic/pharmacodynamic (PK/PD) relationship.
When you need to track very rapid changes in transmitters, fast scan cyclic voltammetry is a great solution. This electrochemical technique enables quantification of monoamine neurotransmitter changes in rodents in real-time, adding further value when combined with other services and techniques as part of a customized program of study.