We employ EEG as a minimally invasive method for recording electrical activity in the brain.
As a leading provider of in vivo preclinical services, we use EEG monitoring to assess changes in sleep state and electrical activity associated with models of CNS disorders. Via telemetry, we can record EEG patterns indicative of changes in underlying neuronal function, and thereby show specific rhythmic activity that can be used as characteristic translational biomarkers moving forward. We can also customize EEG measurements, and use those in combination with other techniques like behavior, microdialysis, and plasma/CSF sample collection to achieve your specific objectives.
Combining EEG with Microdialysis
Our client sought to determine the potential of their compound to induce seizures. Notably, it was critical to determine the free drug levels that induce the changes.
We coupled EEG telemetry with MetaQuant microdialysis, and monitored animals over a few days for seizure activity.
Determining an exact free concentration of a compound that induces subtle seizures.
We were able to identify a dose of compound that was previously not identified as a seizure-inducing dose. This guidance and translatability of EEG was carried forward into human studies.
Identifying non-invasive clinical biomarkers
Client sought to identify non-invasive clinical biomarkers related to their compounds' impact on the brain.
We combined microdialysis with EEG to determine how specific changes in neurotransmitters affected electrical activity in the brain.
Understanding the relationship between pharmacodynamic changes in the brain, and their effect on brain activity, is critical in identifying non-invasive clinical biomarkers. Identifying the neurotransmitter changes that affected brain activity was key, and previously not well understood within the same animal.
We found that administration of our client's compounds induced changes in certain neurotransmitters, and a resultant change in EEG activity, that could then be monitored in future clinical studies.
Understanding sleep patterns is essential in drug development. With our telemetry approaches, we can continuously monitor sleep activity, and the effects of novel therapeutics on the patterns.
- Quantitative EEG with spectral analysis
- Slow wave sleep (SWS)/(NREM sleep)
- Paradoxical sleep (PS)/(REM sleep)
- Acute monitoring
- Chronic monitoring
Seizures are a hallmark of a dysfunctional brain. They can be caused by brain trauma, induction of noxious chemicals, or in some cases therapeutic agents, and by disorders such as epilepsy. This makes understanding seizure activity essential to understanding brain function, and the function of novel therapeutics. We use telemetry, alone or in combination with other techniques such as simultaneous video tracking, to monitor seizure activity.
- Acute seizure detection
- Chronic seizure monitoring
- Simultaneous video tracking
- Behavioral scoring
- Combination with microdialysis
Understanding the effects of test compounds on the activity and function of the brain are essential in drug development. We are able to perform continuous EEG following acute or chronic compound treatment.
- Acute treatment
- Chronic treatment
- Combination with PK, PD, or behavior