Our deep experience supports a full suite of behavioral techniques to cover the key CNS disease domains of Cognition, Mood disorders, Psychosis and Neurodegeneration.
Beyond these stand-alone services, our broad expertise allows for flexible, customized combinations of behavioral assessments with real-time in vivo microdialysis, and/or EEG monitoring. By simultaneously monitoring behaviors and neurochemical changes in the same animals, we offer added value and efficiency by reducing the number of animal samples, and the need for multiple experiments.
Combining Microdialysis and Behavior
Client needed to understand the mechanism of action, and behavioral actions, of a novel compound targeting Alzheimer’s treatment. The compound clearly modified key proteins in vitro, but what about in vivo in animals?
Our customized study program combined push-pull microdialysis with measures of behavior in transgenic mice.
To understand changes in key proteins, antibody levels, and cognitive function in the transgenic mice.
Following repeat treatment with the novel antibody, pathologic Alzheimer's protein levels were decreased, as treatment antibody levels increased, and a clear improvement in cognitive function was observed in the animals. This pivotal data enabled the client to continue with the development of their novel agent.
Anxiety / Depression
We provide a full range of assays used in the development of compounds to treat anxiety and mood disorders, including the ‘gold standard’ assays that show high predictive validity, as well as newer approaches that are being researched and identified.
- Open Field
- Light dark box
- Elevated Plus Maze
- Novelty Suppressed Feeding
- Tail suspension
- Forced swim
Our broad selection of assays associated with aspects of cognition can be used to assess the effects of compounds on various components of learning and memory formation, as well as assessing both pro-cognitive and amnesiac actions of compounds. We often tailor custom protocols and assays to meet precise client needs and requirements.
- Novel object recognition (natural, scop/PCP)
- Contextual/Cue fear condition
- Y-Maze (spontaneous alternation)
- 5 Choice Serial Reaction Time (5CSRTT)
To expedite development of compounds to treat schizophrenia and related disorders, we offer a variety of assays, including a number of well-established ones related to dopaminergic or hypo-glutamatergic hyperactivity. Compounds can also be assessed in the conditioned avoidance assay, as selective disruption of the conditioned response is a characteristic property of both typical and novel antipsychotic agents. As with all of our services, we work to design a program that is specific to client objectives.
- Amphetamine/PCP induced locomotor activity
- Apomorphine induced rearing climbing
- Conditioned Avoidance Response
Neurodegeneration / Movement Disorders
We provide a number of neurodegeneration / movement disorder models, specifically within the area of Parkinson’s disease our range includes utilizing neurotoxic lesions, vector-delivered alpha-synuclein deficits, transgenic animals as well as client-provided animals. Additionally, we can bring in commercially available animal models of other disorders such as Huntington’s, and Alzheimer’s. Our flexible approach to experimental design is built for maximum value and efficiency throughout.
Neurodegeneration and extrapyramidal models include:
- 6-OHDA Lesion
- aSyn AAV Model
- Quinolinic acid lesions
- Transgenic animals (commercially available and client provided)
- Cylinder test or rotational behavior
- L-Dopa induced dyskinesia (AIMs)
- PD Psychosis
- Haloperidol-induced catalepsy
Our complete translational biomarker solution for pain research combines standard behavioral readouts with microdialysis to measure both endogenous biomarkers and compound levels.
If you need to develop an overall behavioral profile for a specific compound, our assays, based on automated and observational measures, can assess motor function, activity, and other behavioral changes in a number of ways. We can design the assays to assess the compound on its own, or in combination with other agents that induce a deficit or hyperactive state, depending on your needs and objectives.
- Locomotor activity
- Observational battery
- Grip strength
- Cylinder test
- Substance Abuse assessment
- CNS safety pharmacology (Irwin testing)